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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Western for IgA

Western for IgA - Western Blot Forum

Western for IgA - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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  #1  
Old 04-03-2013, 07:49 PM
Pipette Filler
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Default Western for IgA



hey

I'm running a Western to determine the presence of IgA in vaginal lavage samples from mice...running on a NuPAGE bis/tris 4-12% gel, with reducing (BME and DTT both) SDS sample buffer. Anyone know where the banding should appear?

Thanks!
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Old 04-05-2013, 02:30 AM
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Default Re: Western for IgA

make sure you have a protein ladder included and compare against
[Only registered users see links. ]
your should be in reduced condition so two band shall be seen.
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Old 04-05-2013, 02:42 PM
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Default Re: Western for IgA

thanks!
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