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sittingchair 04-03-2013 07:49 PM

Western for IgA
 
hey

I'm running a Western to determine the presence of IgA in vaginal lavage samples from mice...running on a NuPAGE bis/tris 4-12% gel, with reducing (BME and DTT both) SDS sample buffer. Anyone know where the banding should appear?

Thanks!

butters 04-05-2013 02:30 AM

Re: Western for IgA
 
make sure you have a protein ladder included and compare against
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your should be in reduced condition so two band shall be seen.

sittingchair 04-05-2013 02:42 PM

Re: Western for IgA
 
thanks!


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