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| Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures. |
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#1
03-05-2013, 03:05 PM
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 Mouse Liver Actin Blot Hi, 1st time poster, long time lurker. I've recently been doing immunoblotting on mouse liver lysates. I'm using an antibody cocktail of my 2 target proteins and my loading control: beta-actin. The problem is that the beta-actin is coming out as a triple band. All very close to each other, but far enough to resolve. The bands are also of equal intensity. I can't figure out what's going on here and after frantic google searching I've come here, hoping to see if anyone has an answer. Thanks  |
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#2
03-06-2013, 12:59 AM
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| Re: Mouse Liver Actin Blot It would be wonderful if you have the picture that goes with it. Thanks! |
| The Following User Says Thank You to butters For This Useful Post: | ||
admin (03-07-2013)
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#3
03-06-2013, 10:13 AM
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| Re: Mouse Liver Actin Blot |
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| Tags |
| actin , blot , liver , mouse |
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