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Mouse Liver Actin Blot

Mouse Liver Actin Blot - Western Blot Forum

Mouse Liver Actin Blot - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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  #1  
Old 03-05-2013, 04:05 PM
Pipette Filler
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Default Mouse Liver Actin Blot



Hi,
1st time poster, long time lurker. I've recently been doing immunoblotting on mouse liver lysates. I'm using an antibody cocktail of my 2 target proteins and my loading control: beta-actin.

The problem is that the beta-actin is coming out as a triple band. All very close to each other, but far enough to resolve. The bands are also of equal intensity. I can't figure out what's going on here and after frantic google searching I've come here, hoping to see if anyone has an answer.

Thanks
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Old 03-06-2013, 01:59 AM
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Default Re: Mouse Liver Actin Blot

It would be wonderful if you have the picture that goes with it. Thanks!
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Old 03-06-2013, 11:13 AM
Pipette Filler
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Default Re: Mouse Liver Actin Blot

Hi, here's two shots of the actin band attached[Only registered users see links. ]
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actin , blot , liver , mouse


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