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-   -   Mouse Liver Actin Blot (http://www.molecularstation.com/forum/western-blot-forum/87690-mouse-liver-actin-blot.html)

griddlelol 03-05-2013 04:05 PM

Mouse Liver Actin Blot
 
Hi,
1st time poster, long time lurker. I've recently been doing immunoblotting on mouse liver lysates. I'm using an antibody cocktail of my 2 target proteins and my loading control: beta-actin.

The problem is that the beta-actin is coming out as a triple band. All very close to each other, but far enough to resolve. The bands are also of equal intensity. I can't figure out what's going on here and after frantic google searching I've come here, hoping to see if anyone has an answer.

Thanks

butters 03-06-2013 01:59 AM

Re: Mouse Liver Actin Blot
 
It would be wonderful if you have the picture that goes with it. Thanks!

griddlelol 03-06-2013 11:13 AM

Re: Mouse Liver Actin Blot
 
1 Attachment(s)
Hi, here's two shots of the actin band attached[Only registered and activated users can see links. Click Here To Register...]


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