| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
02-15-2013, 10:03 AM
| |||||||||||
| |||||||||||
 Stronger signal after stripping my western blot Hi, since the end of last year, western blotting is giving me a hard time. After (first time) antibody incubation of my western blot I always get a weak signal of my wanted protein band. However after stripping my blot and incubation with the same protocol and antibodies I get a stronger signal. Before (mid of last year and earlier) I did not have this problem. I did not change my protocol, checked everything and just don't know why it is this way. Does somebody have the same problem or knows what the problem is? Thanks a lot in advance.  |
|
| Tags |
| blot , signal , stripping , stronger , western |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Western Blot Gel Trend Increasing Signal from One Side to Other? | preethi | Western Blot Forum | 2 | 12-31-2011 10:30 PM |
| Western Blot Stripping Blots | isabelle | Western Blot Forum | 3 | 03-31-2011 07:54 PM |
| Western Blot Membrane Stripping Reprobing Disadvantages | trudy | Western Blot Forum | 1 | 03-31-2011 07:40 PM |
| Western Blot Background Troubleshooting | molecule2005 | Western Blot Forum | 18 | 10-07-2010 11:49 AM |
| dot blot and western blot | Liang | Western Blot Forum | 1 | 08-07-2009 03:26 PM |
Linear Mode
