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Stronger signal after stripping my western blot

Stronger signal after stripping my western blot - Western Blot Forum

Stronger signal after stripping my western blot - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 02-15-2013, 11:03 AM
Pipette Filler
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Question Stronger signal after stripping my western blot



Hi,

since the end of last year, western blotting is giving me a hard time. After (first time) antibody incubation of my western blot I always get a weak signal of my wanted protein band. However after stripping my blot and incubation with the same protocol and antibodies I get a stronger signal.

Before (mid of last year and earlier) I did not have this problem. I did not change my protocol, checked everything and just don't know why it is this way.

Does somebody have the same problem or knows what the problem is?

Thanks a lot in advance.
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