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#1
01-15-2013, 12:54 PM
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 western blot troubleshooting hi everyone, I'm a new PhD student in France, and it's the first time I post a question on a scientific forum. I'm working on apoptosis in human cells MDAMB231, MCF7 and HUVEC. And I've got a big problem on my western blot results. I add two exemples of results when I'm looking for actin. I'v already try to denaturate again my samples in LAEMNLI solution 5mn at 100°C (test 080112) and it's a little bit better but not perfect. Have you got any idea to explain those results ? Thanks a lot Laetitia  |
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