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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


western blot troubleshooting

western blot troubleshooting - Western Blot Forum

western blot troubleshooting - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 01-15-2013, 01:54 PM
Pipette Filler
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Question western blot troubleshooting



hi everyone,

I'm a new PhD student in France, and it's the first time I post a question on a scientific forum.

I'm working on apoptosis in human cells MDAMB231, MCF7 and HUVEC. And I've got a big problem on my western blot results.
I add two exemples of results when I'm looking for actin.

I'v already try to denaturate again my samples in LAEMNLI solution 5mn at 100C (test 080112) and it's a little bit better but not perfect.

Have you got any idea to explain those results ?

Thanks a lot

Laetitia
Attached Images
File Type: jpg test 080112 ecl+1.jpg (9.4 KB, 1 views)
File Type: jpg actine ecl+ mda huvec.jpg (18.2 KB, 1 views)
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