Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Western Blot Forum (http://www.molecularstation.com/forum/western-blot-forum/)
-   -   western blot troubleshooting (http://www.molecularstation.com/forum/western-blot-forum/87322-western-blot-troubleshooting.html)

laetitiaN 01-15-2013 01:54 PM

western blot troubleshooting
 
2 Attachment(s)
hi everyone,

I'm a new PhD student in France, and it's the first time I post a question on a scientific forum.

I'm working on apoptosis in human cells MDAMB231, MCF7 and HUVEC. And I've got a big problem on my western blot results.
I add two exemples of results when I'm looking for actin.

I'v already try to denaturate again my samples in LAEMNLI solution 5mn at 100C (test 080112) and it's a little bit better but not perfect.

Have you got any idea to explain those results ?

Thanks a lot

Laetitia


All times are GMT. The time now is 11:42 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08882 seconds with 11 queries