western blot troubleshooting
 

hi everyone,

I'm a new PhD student in France, and it's the first time I post a question on a scientific forum.

I'm working on apoptosis in human cells MDAMB231, MCF7 and HUVEC. And I've got a big problem on my western blot results.
I add two exemples of results when I'm looking for actin.

I'v already try to denaturate again my samples in LAEMNLI solution 5mn at 100°C (test 080112) and it's a little bit better but not perfect.

Have you got any idea to explain those results ?

Thanks a lot

Laetitia