western blot troubleshooting
I'm a new PhD student in France, and it's the first time I post a question on a scientific forum.
I'm working on apoptosis in human cells MDAMB231, MCF7 and HUVEC. And I've got a big problem on my western blot results.
I add two exemples of results when I'm looking for actin.
I'v already try to denaturate again my samples in LAEMNLI solution 5mn at 100°C (test 080112) and it's a little bit better but not perfect.
Have you got any idea to explain those results ?
Thanks a lot
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