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Tris-Glycine and Tris-HEPES question

Tris-Glycine and Tris-HEPES question - Western Blot Forum

Tris-Glycine and Tris-HEPES question - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 10-16-2012, 03:15 PM
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Default Tris-Glycine and Tris-HEPES question



Hey all,

I know you can't run Tris-HEPES gels with Tris-Glycine-SDS running buffer, but can you run Tris-Glycine gels with Tris-HEPES-SDS running buffer?

Thanks!!
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Old 10-17-2012, 11:01 AM
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Default Re: Tris-Glycine and Tris-HEPES question

Precast gel from Invitrogen (Novex) is made with Tris-Glycine buffer, while gel from Thermo Scientific Pierce is produced with Tris-HEPES buffer, both for homogeneous and gradient electrophoresis. Both gels do not contain SDS that is incorporated in the run buffer. Run nonreducing buffers from you described Tris-HEPES-SDS (10x : 121 g/L Tris base, 238 g/L HEPES and 10/L g SDS at pH pH 8.0 0.25) or Tris-Glycine-SDS (10x : 29 g/L Tris, 144 g/L Glycine and 10 g/L SDS at pH 8.3), used in 1x form, they is compatible with both gels but each buffer produces different migration patterns.
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