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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


antibody incubation

antibody incubation - Western Blot Forum

antibody incubation - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 08-13-2012, 11:39 AM
Pipette Filler
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Default antibody incubation



Hello everybody,

I am trying to study the expression of calpain and disferlin by western blot. I performed a first incubation with tubulin and calpain, but I only obtained hybridization of tubulin. Before the stripping of the membrane to incubate again with calpain, I would like to incubate the membrane with disferlin but I donīt know if I have to incubate again with tubulin or just using disferlin.

Thank you!
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