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#1
08-09-2012, 08:34 AM
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 How to extend the signal duration of Homemade ECL I have made the homemade ECL like this: Solution A (50ml): 50 ml 1mM Tris pH 8.5 110 ul 90 mM coumaric acid (0.2mM coumaric acid) 250 ul 250 mM Luminol (final 1.25mM Luminol) Solution B (50ml) 50ml 100mM Tris 8.6 35ul 30% H2O2 To use, mix 500ul Solution A and Solution B. I found this homemade ecl could generate signal with a short duration (less than 3mins). How could I extend the duration? thanks!  |
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| duration , ecl , extend , homemade , signal |
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