Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Western Blot Forum
Register Search Today's Posts Mark Forums Read

Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


How to extend the signal duration of Homemade ECL

How to extend the signal duration of Homemade ECL - Western Blot Forum

How to extend the signal duration of Homemade ECL - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 08-09-2012, 08:34 AM
Pipette Filler
Points: 54, Level: 1 Points: 54, Level: 1 Points: 54, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2012
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Question How to extend the signal duration of Homemade ECL



I have made the homemade ECL like this:

Solution A (50ml):
50 ml 1mM Tris pH 8.5
110 ul 90 mM coumaric acid (0.2mM coumaric acid)
250 ul 250 mM Luminol (final 1.25mM Luminol)

Solution B (50ml)
50ml 100mM Tris 8.6
35ul 30% H2O2

To use, mix 500ul Solution A and Solution B.

I found this homemade ecl could generate signal with a short duration (less than 3mins). How could I extend the duration? thanks!
Reply With Quote
Reply

Tags
duration , ecl , extend , homemade , signal


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Dose dependent increase in signal with serial dilutions? 2046 ELISA Assay Forum 2 05-02-2012 08:05 AM
Problems with signal in WB New_PhD Western Blot Forum 2 02-17-2011 05:12 PM
Acceleration = direction, or (velocity-magnitude) / duration, change. brian a m stuckless Physics Forum 0 10-31-2005 09:15 AM
New transistor laser could lead to faster signal processing - incorporating quantum wells... Ken Kubos Physics Forum 0 11-17-2004 06:53 PM


All times are GMT. The time now is 09:10 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10929 seconds with 16 queries