How to extend the signal duration of Homemade ECL
I have made the homemade ECL like this:
Solution A (50ml):
50 ml 1mM Tris pH 8.5
110 ul 90 mM coumaric acid (0.2mM coumaric acid)
250 ul 250 mM Luminol (final 1.25mM Luminol)
Solution B (50ml)
50ml 100mM Tris 8.6
35ul 30% H2O2
To use, mix 500ul Solution A and Solution B.
I found this homemade ecl could generate signal with a short duration (less than 3mins). How could I extend the duration? thanks!
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