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What happens if you leave lysis out in room temperature for a long time?

What happens if you leave lysis out in room temperature for a long time? - Western Blot Forum

What happens if you leave lysis out in room temperature for a long time? - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 08-03-2012, 11:31 PM
Pipette Filler
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Default What happens if you leave lysis out in room temperature for a long time?



I had lysis left out in room temperature for about 2-4 hours after thawing. Is there going to be a detection problem for phospho protein, especially pAKT and p4EBP1, the next time I run these samples? Or am I forced to create a new batch of lysis?


Thanks in advance for your replies.
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