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-   -   What happens if you leave lysis out in room temperature for a long time? (http://www.molecularstation.com/forum/western-blot-forum/86172-what-happens-if-you-leave-lysis-out-room-temperature-long-time.html)

euphoric.symphony 08-03-2012 11:31 PM

What happens if you leave lysis out in room temperature for a long time?
 
I had lysis left out in room temperature for about 2-4 hours after thawing. Is there going to be a detection problem for phospho protein, especially pAKT and p4EBP1, the next time I run these samples? Or am I forced to create a new batch of lysis?


Thanks in advance for your replies.


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