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| A less harsh (and stench-free :-)), but VERY efficient alternative: wash blot for 5 min in distilled water immerse in 0.2M NaOH and wash for 5 minutes wash again for 5 minutes in distilled water Block for 30-60 min If any trace of signal still remains you can raise the concentration of NaOH up to 2M and the wash time up to 30 min (although I 've never needed to...). |
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| 0.2 M NaOH isn't really very gentle either. I'm busy (lazy?), so I buy Restore stripping buffer from Pierce. It doesn't have the odor of mercaptoethanol and I haven't had problems with losing signal because it's too harsh. |
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