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Storing PVDF membrane for more than 3 weeks

Storing PVDF membrane for more than 3 weeks - Western Blot Forum

Storing PVDF membrane for more than 3 weeks - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 05-08-2012, 12:10 AM
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Default Storing PVDF membrane for more than 3 weeks



Hi everyone,

I am new to Western blotting and I was wondering what is the best way to store a PVDF membrane that I already probed once with an antibody. I want to reprobe the membrane with another antibody but I will be away from the lab for a prolonged period. I am currently storing it in 1X TBS in 4degrees. I also have not stripped the membrane of the antibodies from the last western yet. Any suggestions?
Thanks!
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