|Register||Search||Today's Posts||Mark Forums Read|
|Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.|
| ||LinkBack||Thread Tools||Display Modes|
Western blot smudgy, scraggly bands
Hello, this is my first time posting. I have a question as to what happened here on this blot especially at the bottom.
We loaded 100ug of protein and I was wondering if this was a result of too much. We loaded max total into premade bis-tris gel.
When it ran, it was very smeary and blobby as well.
I've attached a photo. I apologize for the horrible quality.
Re: Western blot smudgy, scraggly bands
Probably. Try 40ug. If that doesn't work please post your protocol.
|bands , blot , scraggly , smudgy , western|
|Thread||Thread Starter||Forum||Replies||Last Post|
|Western blot with more strong bands||biotom||Western Blot Forum||3||09-08-2011 01:49 PM|
|Western Blot Mystery - Developed bands don't adhere to membrane||cyrus||Western Blot Forum||4||01-20-2009 08:08 PM|
|Way too many bands on western blot||es0teric||Western Blot Forum||1||09-19-2008 11:54 AM|
|Western Blot - Extra Bands||moleculardude||Western Blot Forum||3||10-17-2006 03:24 PM|