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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Western Blot - Vertical Streaking

Western Blot - Vertical Streaking - Western Blot Forum

Western Blot - Vertical Streaking - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 04-18-2012, 02:21 PM
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Default Western Blot - Vertical Streaking



Hi I was wondering if anyone could help me. I have been trying to runa western for the NLRP3 protein which makes up the inflammasome. However mostly all im getting are vertical streaks (see pic)

The samples are supernatants from cultured macrophages lysed in m-TER (Thermo Scientific) and the precipitated from acetone and dissolved in 1X laemlli buffer!

I have ran a few times but nothing seems to correct it. I am using a NuPAGE system

Thanks

ashleigh
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File Type: jpg western ponceau NLRP3.jpg (45.2 KB, 12 views)
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