I tried to detect protein with western blotting. previously, my colleague did the experiment and it worked. but when I applied the procedure to different cell organs, the result was not good, eventhough it was detectable.
despite my low skills as consideration, I wonder does cells/organ type give credits to the results (high backgroud, unspecific band, etc)?
specifically, is there any specific consideration for protein extraction from liver organs?
Thank you for your suggestion