I'm a new user of iBlot transfer system and I have a question for those who have much experience than I do
. I've just tried to transfer proteins from a gel to a Nitrocellulose membrane using iBlot kit and apparently it works perfect since the ladder has completely transferred
. However, after I disassemble the iBlot components (removing the sponge, the gel,...) I think I was slow enough so the membrane dried before moving it to the blocking buffer
! So is that going to affect the Ab binding
Thanks for your help.