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iBlot Nitrocellulose membrane dried!
I'm a new user of iBlot transfer system and I have a question for those who have much experience than I do . I've just tried to transfer proteins from a gel to a Nitrocellulose membrane using iBlot kit and apparently it works perfect since the ladder has completely transferred . However, after I disassemble the iBlot components (removing the sponge, the gel,...) I think I was slow enough so the membrane dried before moving it to the blocking buffer ! So is that going to affect the Ab binding ?
Thanks for your help.
Re: iBlot Nitrocellulose membrane dried!
No, i don't think there is any need to worry about it.
And the blot not going to dry completely even if you left it for 1 hr since it always holds little fluidity. Even it is completely dry don't worry you will get good result.
Once in our lab we received a blot from other lab (completely dry state) to test few proteins and it worked. So go ahead with your western Blot.
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|dried , iblot , membrane , nitrocellulose|
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