iBlot Nitrocellulose membrane dried!

[fmhakami]

Hi ,

I'm a new user of iBlot transfer system and I have a question for those who have much experience than I do . I've just tried to transfer proteins from a gel to a Nitrocellulose membrane using iBlot kit and apparently it works perfect since the ladder has completely transferred . However, after I disassemble the iBlot components (removing the sponge, the gel,...) I think I was slow enough so the membrane dried before moving it to the blocking buffer ! So is that going to affect the Ab binding ?

Thanks for your help.

Fahad

[obama]

No, i don't think there is any need to worry about it.

And the blot not going to dry completely even if you left it for 1 hr since it always holds little fluidity. Even it is completely dry don't worry you will get good result.

Once in our lab we received a blot from other lab (completely dry state) to test few proteins and it worked. So go ahead with your western Blot.