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Can I use the same nitrocellulose membrane twice for protein transfer?

Can I use the same nitrocellulose membrane twice for protein transfer? - Western Blot Forum

Can I use the same nitrocellulose membrane twice for protein transfer? - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 10-13-2011, 06:28 PM
Pipette Filler
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Default Can I use the same nitrocellulose membrane twice for protein transfer?



Hi,

I had a mistake during the first time transfer: the electrodes were reversed so I got nothing on the blot. Of cause I am going to run my protein samples again. But I just wonder if that same nitrocellulose membrane can be used again for transfer ?

Hope someone can help with the issue. Thanks!

Hai
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Old 12-14-2011, 06:21 AM
Pipette Filler
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Default Re: Can I use the same nitrocellulose membrane twice for protein transfer?

Sure you can use it..But for better results use new blot..
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membrane , nitrocellulose , protein , transfer


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