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Immunoprecipitated samples

Immunoprecipitated samples - Western Blot Forum

Immunoprecipitated samples - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 09-07-2011, 11:09 PM
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Default Immunoprecipitated samples



A protein that I immunoprecipitated from cell lysate, runs a little bit higher than the expected size. Has any one observed IP samples running at incorrect molecular weights on a 10% SDS-polyacrylamide gel?
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Old 09-08-2011, 07:13 AM
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Default Re: Immunoprecipitated samples

it will easy to answer, if you mention what was the expected size of your band and where you getting the band.
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Old 09-08-2011, 04:58 PM
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Default Re: Immunoprecipitated samples

Thanks for the quick response. The expected size is ~95 kDa; the immunoprecipitated band elutes between the 95 kDa and 130 kDa bands of the protein ladder.

Thanks!
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Old 09-09-2011, 11:06 AM
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Default Re: Immunoprecipitated samples

what type of protein is that, if it is glycoprotein. some time after immunoprecipitation and other process leads to some changes in such protein. there by changing it charge or some even MW of such protein because (carbohydrate moity in it). I think this shift may be because of that...
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Old 09-13-2011, 05:44 PM
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Default Re: Immunoprecipitated samples

Thanks for your input!
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