| | |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures. |
| | LinkBack | Thread Tools | Display Modes |
|
#1
| |||||||||||
| |||||||||||
| What can I do to intensify the bands of my western blot? I managed to get a good result for protein and need to repeat the result for the other two. I make the revelation in the nitrocellulose membrane with DAB and cloronaftol. I try to use streptavidin secondary antibody before? Thanks |
|
#2
| |||||||||||
| |||||||||||
| Hello all! I forget to introduce myself! My name is Regiana and i work with bats. I am very happy to have found a forum for discussion on western blot. I'm having problems with my experiment ... Can someone help me with the question above? Thanks in advance! |
|
#3
| ||||||||||||
| ||||||||||||
| You can use Boost reagent instead of Milk solution. Ie what we use to intensify the week bands in western Blot |
|
#4
| |||||||||||
| |||||||||||
| Thank you for the your answer. You helped me so much. |
| Tags |
| bands , blot , strong , western |
| Thread Tools | |
| Display Modes | |
|
|
| | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Western Blot Mystery - Developed bands don't adhere to membrane | cyrus | Western Blot Forum | 4 | 01-20-2009 08:08 PM |
| Way too many bands on western blot | es0teric | Western Blot Forum | 1 | 09-19-2008 11:54 AM |
| Absence of Bands on Western Blot Film | maprinzz | Western Blot Forum | 3 | 09-12-2008 04:26 PM |
| Western Blot - Extra Bands | moleculardude | Western Blot Forum | 3 | 10-17-2006 03:24 PM |
| Nobel Prize for David Thomson?! | caltechdude | Physics Forum | 428 | 01-21-2005 09:39 PM |