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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Western blot with more strong bands

Western blot with more strong bands - Western Blot Forum

Western blot with more strong bands - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 09-07-2011, 02:06 PM
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Default Western blot with more strong bands



What can I do to intensify the bands of my western blot?
I managed to get a good result for protein and need to repeat the result for the other two.
I make the revelation in the nitrocellulose membrane with DAB and cloronaftol. I try to use streptavidin secondary antibody before?

Thanks
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Old 09-07-2011, 02:15 PM
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Default Re: Western blot with more strong bands

Hello all!
I forget to introduce myself! My name is Regiana and i work with bats. I am very happy to have found a forum for discussion on western blot. I'm having problems with my experiment ... Can someone help me with the question above?
Thanks in advance!
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Old 09-07-2011, 06:25 PM
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Default Re: Western blot with more strong bands

You can use Boost reagent instead of Milk solution. Ie what we use to intensify the week bands in western Blot
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Old 09-08-2011, 01:49 PM
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Smile Re: Western blot with more strong bands

Thank you for the your answer. You helped me so much.


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