Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Western Blot Forum
Register Search Today's Posts Mark Forums Read

Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Western Blot Gel Trend Increasing Signal from One Side to Other?

Western Blot Gel Trend Increasing Signal from One Side to Other? - Western Blot Forum

Western Blot Gel Trend Increasing Signal from One Side to Other? - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-12-2006, 09:41 PM
Pipette Filler
Points: 1,723, Level: 25 Points: 1,723, Level: 25 Points: 1,723, Level: 25
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2006
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Cool Western Blot Gel Trend Increasing Signal from One Side to Other?



Hi
We have problems with our westerns. We see a trend in our western image from left to right. There is a increase in signal in bands from left to right. We tried different antibodies and confirmed that there is no problem with antibodies. We loaded constant amount of protein, but still we see the trend. We run gels in Bio-Rad gel apparatus. Transferring is done in Semi dry Transfer gel Apparatus at 20 volts for 1hr & 15 minutes. The memberanes are developed using Femto developing solution. Can you give us suggestions,

best regards
Reply With Quote
  #2  
Old 09-06-2011, 03:48 AM
admin's Avatar
Administrator
 
Join Date: Nov 2005
Posts: 1,418
Thanks: 883
Thanked 68 Times in 58 Posts
Default Re: Western Blot Gel Trend Increasing Signal from One Side to Other?

I would try another/new ladder, but also re-do buffers and the gel. May be interference between the ladder and the developing steps
Reply With Quote
  #3  
Old 12-31-2011, 11:30 PM
Pipette Filler
Points: 20, Level: 1 Points: 20, Level: 1 Points: 20, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2011
Posts: 5
Thanks: 3
Thanked 1 Time in 1 Post
Default Re: Western Blot Gel Trend Increasing Signal from One Side to Other?

You can try different transferring system. That might help~!
Reply With Quote
Reply

Tags
blot , gel , increasing , side , signal , trend , western


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Western Blot Background Troubleshooting molecule2005 Western Blot Forum 18 10-07-2010 11:49 AM
Can we use dot blot inplace of Western blot? peterish Western Blot Forum 3 06-16-2010 07:59 PM
dot blot and western blot Liang Western Blot Forum 1 08-07-2009 03:26 PM
effexor side effects sweating zocor effects on gallbladder sideeffects expired soma chelsea6breasts@gmail.com Cell Biology and Cell Culture 0 03-27-2008 12:14 PM


All times are GMT. The time now is 01:33 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12393 seconds with 16 queries