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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Ladder problem

Ladder problem - Western Blot Forum

Ladder problem - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 08-21-2011, 01:15 AM
Pipette Filler
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Default Ladder problem



Does anyone recommend a ladder with a range that encompasses 30kd to 200kd? The one I've been using does not show up in my image. After electrophoresis, I transfer the proteins to nitrocellulose and my secondary is anti-rabbit HRP. I add super signal just before imaging with a UVP system. The ladder than I use does not show up in the image but it did when I used PVDF, ECL, and a typhoon imager. Interestingly, this ladder only reacts with one of my primary antibodies and that's it. Not sure what is going on! Thanks!
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