Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Western Blot Forum
Register Search Today's Posts Mark Forums Read

Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Yellow precipitation in Licor Blocking buffer

Yellow precipitation in Licor Blocking buffer - Western Blot Forum

Yellow precipitation in Licor Blocking buffer - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 08-04-2011, 09:20 PM
Pipette Filler
Points: 35, Level: 1 Points: 35, Level: 1 Points: 35, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Red face Yellow precipitation in Licor Blocking buffer



Has anybody used Licor Blocking Buffer? I have been reusing Licor Blocking buffer but noticed that there is some yellowish precipitation on the bottom. If I shake it, it seems to dissolve in the solution. What could this be? Will it affect the blocking?
Reply With Quote
  #2  
Old 09-01-2011, 10:28 PM
Pipette Filler
Points: 62, Level: 1 Points: 62, Level: 1 Points: 62, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Yellow precipitation in Licor Blocking buffer

Hello,
LI-COR does not recommend re-using the Blocking Buffer. Always use fresh. There is lot to lot variability with fresh blocking buffer, which may include the yellow precipitation you are seeing. The precipitation is usually lipids that have come out of suspension. Placing it on a gentle shaker for an hour will cause the lipids to go back into suspension. This should not affect the performance of the blocking buffer. Hope this helps!
Reply With Quote
  #3  
Old 09-10-2011, 02:19 PM
Pipette Filler
Points: 146, Level: 2 Points: 146, Level: 2 Points: 146, Level: 2
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2011
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Yellow precipitation in Licor Blocking buffer

We are also using Licor block buffer and sometimes notice the precipitation but it doesn't seem to have an effect on blocking performance. I tend to use fresh buffer for the initial block and then re-use that block buffer diluted 1:1 with PBS for subsequent antibody incubations.
Reply With Quote
Reply

Tags
blocking , blocking buffer , buffer , licor , precipitation , yellow


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
protein purification magnificientleo Proteomics Forum 11 12-29-2010 09:01 AM
SDS-PAGE Protocol moleculardude Protein Science 14 02-12-2010 11:27 AM
Protein precipitation in HPLC buffer Mouldi SAIDI Protein Forum 1 01-23-2009 06:25 PM
Production of Monoclonal Antibodies - Technique in Mouse Cellbiogal Antibody Forum 2 01-08-2009 10:52 PM
Methods Digest, Vol 18, Issue 19 1. Qiagen buffer PN Jess Protocols and Methods Forum 0 11-22-2006 10:45 PM


All times are GMT. The time now is 12:36 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13624 seconds with 16 queries