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-   -   Membrane Incubation Plates/Trays (http://www.molecularstation.com/forum/western-blot-forum/83073-membrane-incubation-plates-trays.html)

sciencegirl11 06-29-2011 03:24 PM

Membrane Incubation Plates/Trays
 
This is a pretty straight forward question. I've looked online for quite awhile and have been unable to find plates/trays used for incubating the membrane that have a lid. I was curious what most of you use. Generally, we use a tip lid, or the box (to have the lid). The problem with this is that it takes nearly 40mL to cover the membrane which is too much. I am looking for something that can hold the membrane and cover it with 10mL. Any suggestions?

Thanks in advance!:happy:

Emily.Koury 08-04-2011 04:02 PM

Re: Membrane Incubation Plates/Trays
 
Using tip tray lids the size of your membrane or using a beaker that is about the size is the most efficient way to submerge the membrane without using a rotisserie or a heat seal bag. Personally- my antibody concentration is 1:10 so I use a rotisserie with the 50 mL disposable tubes and can get away with using only 4-3 mL of solution.

JuanM 02-16-2012 06:30 PM

Re: Membrane Incubation Plates/Trays
 
We use the PerfectWestern Containers from GenHunter. They have 27 different sized Western boxes, so there is always the right size for what you need. For the standard mini gel size [their Medium (Cat. No. B101)], we can use as little as 2-3 mLs per Western. Much better than tip lids or conical tubes.


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