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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


New Gel Doc

New Gel Doc - Western Blot Forum

New Gel Doc - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 03-26-2011, 10:41 AM
Pipette Filler
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Our lab recently acquired a Carestream Gel Logic 2200 PRO. To image our westerns we use horseradish peroxidase-conjugated secondary antibody (HRP; Amersham) and incubate in ECL reagent (Amersham). I have been trying to image these blots using the default Chemi luminescent settings on the machine, but am getting really terrible images (images look fine when exposed on film in dark room). I'm thinking someone may have inadvertently changed the settings for Chemi detection and was wondering if anyone could provide me with the settings that work on their machine to image chemiluminescent westerns.

Thanks!
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amersham , chemilumiescent , doc , ecl , gel , hrp , western


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