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SERCA2a: various bands

SERCA2a: various bands - Western Blot Forum

SERCA2a: various bands - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 03-22-2011, 06:14 PM
Pipette Filler
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Default SERCA2a: various bands



Hiya! I was wondering if anyone has used the anti-serca antibody on rat cardiac homogenates, and how your blots look like when they came out from the developer?
I am using Anti-Serca antibody from Millipore, and I have 'boiled' my samples for 5minutes...Used 9% gel..
Does anyone just get one band at ~ 110KDa? or, like me- get bands at ~110KDa and other bands elsewhere on the blots too? This is confusing me as I am not sure what these other bands are?
Please help me! thanks so much in advance..
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Old 03-16-2012, 01:05 PM
Pipette Filler
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Default Re: SERCA2a: various bands

Hi Sammie! I usually use a-SERCA antibody on rat cardiac homogenates for western blott. When I boiled them for 5, the band on 110kDa dissapear (I have no reasson for that), so I kept them on ice until I put them inside the gel. Even this, same inespecifics bands still appear. I have another problem, maybe you can help. Same samples appear o disappear depending on the experiments although they were prepair in the same conditions.
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