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| Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures. |
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#1
03-14-2011, 12:18 PM
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 Too clear bands in the centre of my WB Hello everybody! I’ve been having several issues with my westerns: These days I’ve been detecting clearer bands in the centre of the membrane while the bands on the sides are ok... Even with the actins or GAPDH..!! Now I can't rely on my results... Does anybody know why is this happening to me?  Last edited by Laura10; 03-14-2011 at 04:08 PM.  |
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#2
04-13-2011, 09:42 PM
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| Re: Too clear bands in the centre of my WB I had this problem for a while, and it was because my sandwiches were packed too tightly and were just "squeezed" better on the ends resulting in a more efficient transfer at the edges. Did you recently switch filter papers or sponges? That's when we caught it. Are you using a stir bar in your transfer? That can be another source of what you're seeing - too much turbulence in the center. I no longer use the stir bar and have no problems with overheating. Best of luck. |
| The Following User Says Thank You to probingkate For This Useful Post: | ||
Laura10 (04-20-2011)
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| bands , centre , clear |
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