This should be quick for someone who has experience. So, someone in my lab tells me I should try to isolate CYP450 from rat brain vessels, without using an extraction buffer (only homogenise tissue to brake the cells, because he says that buffer could interfere with Bradford reagent), and cetrifugation at 13,000 rpm. I think it is impossible because I need 100,000 rpm centrifugation to get soluble proteins in supernatant (free from ER).
Is it possible? Why would I even do this if it's no point in doing that. In every scientific paper they say the same, I need an ultracentrifuge-which I don't have.
(anyone has a spare ultracentrifuge?)