Originally Posted by danfive
Your pic looks fine, I did notice some difference in the lane widths. But that is probably due to running ~17 lanes. That's a lot of samples for this one gel, and that is likely what causing the slight distortions---overall your coomassie stained gel is certainly of acceptable quality.
I wouldn't dilute with water, just a waste, put your serum in sample buffer or Laemmli Buffer.
Hi Danfive and those who may know the answer,
I am doing Western Blot with plasma samples.
I am facing with the problem of too concentrated my plasma sample until 80 ug/ul.
I need to dilute it with Laemmli Buffer?
Using 1 X Laemmli?
Thanks for your kindly attention.