How to Run a Concentrated Protein Sample for Western Blot?
I am doing Western Blot with plasma samples.
I am facing with the problem of too concentrated my plasma sample until 80 ug/ul.
I need to dilute it with Laemmli Buffer?
Using 1 X Laemmli?
Thanks for your kindly attention.
Re: How to Run a Concentrated Protein Sample for Western Blot?
depending on if you want a more concentrated sample - you can simply lyse your cells in loading or Laemmli Buffer directly. Then load the amount you need.
If you want to run diluted samples, you can simply analyze your samples to find protein concentration - use the Bradford Assay or a similar kit. Then simply dilute the samples accordingly. You can use Laemmli Buffer or loading buffer to do this.
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