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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Which is my band???

Which is my band??? - Western Blot Forum

Which is my band??? - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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  #1  
Old 05-13-2010, 12:55 PM
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Default Which is my band???



Hi,
I'm performing westerns to detect IkBalpha and p-IkBalpha, but I have problems in identifying the right band, also because sometimes I didn't see the standard...could you help me please?????
I attach my blots!
Thank you everybody!

PS The molecular wheight indicated in the data sheet is 37kDa!
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File Type: jpg GA2 B IkBalpha (12.05.10)30.jpg (81.2 KB, 34 views)
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  #2  
Old 05-13-2010, 04:57 PM
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Default Re: Which is my band???

What is supposed to be in each lane? Are any of these negative controls? You really need a negative control to decide whether any of the bands are real.
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Old 05-14-2010, 08:03 AM
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Default Re: Which is my band???

No, unfortunately I haven't negative and positive controls...this is a big problem!!!
Thank you for your answer!
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Old 05-19-2010, 09:18 PM
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Default Re: Which is my band???

Is the first lane a ladder...what is the scale of the ladder supposed to be? you said your band of interest was around 37kDa...do you have apparent weights for the 2 bands that you do see?
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