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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Best PVDF Membrane

Best PVDF Membrane - Western Blot Forum

Best PVDF Membrane - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 04-20-2010, 08:21 PM
Pipette Filler
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Default Best PVDF Membrane



Hey everyone,

I was just wondering what PVDF membrane people use. What do you like/not like about it?

We use the Bio-Rad 0.2um and my PI is wondering if it might be the reason for "bald spots" in blotting.

Thanks!
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Old 04-25-2010, 09:04 PM
Pipette Filler
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Default Re: Best PVDF Membrane

0.2um is pretty small pore size if you're looking for your proteins in a normal size range. Small pore size = poor transfer of anything but rather small proteins. We use the .45um from Millipore and it works pretty well for us. One time we used .2um by mistake and our blots were terrible so going up to something like .45um might not be a bad idea.
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