Originally Posted by mai-mb
This helped me so much!! Though I don't get a transfer unless I run it for 1:47 mins at 10volts.....
using E-C 150 and the amps hang out around 100.
what am i doing wrong?
Hi, I'm not sure what you mean.
Do you run 1hour 47 minutes at 10 volts or 1.47min?
What is EC 150?
Is everything completely wet/soaked with tranfer buffer?
Are you sure the membrane is activated and equilibrated with transfer buffer?
(1 min or less in Methanol, 1 minute in MQ H20 while on shaker, 15 minutes or more in tranfer buffer while on platform shaker)
How much protein/lane are you trying to tranfer?