Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Western Blot Forum
Register Search Today's Posts Mark Forums Read

Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


DR5 (TRAIL-R1) Antibody - MCF-7 cells - multiple bands

DR5 (TRAIL-R1) Antibody - MCF-7 cells - multiple bands - Western Blot Forum

DR5 (TRAIL-R1) Antibody - MCF-7 cells - multiple bands - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-26-2010, 09:17 PM
Pipette Filler
Points: 806, Level: 16 Points: 806, Level: 16 Points: 806, Level: 16
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2010
Posts: 2
Thanks: 0
Thanked 1 Time in 1 Post
Default DR5 (TRAIL-R1) Antibody - MCF-7 cells - multiple bands



When performing a western blot on MCF-7 whole cell lysates I see a prominent band at around 37kDa and theree addl bands above it, the last one around 50kDa. However, when I run a 293 cell whole cell lysate (harvested the same way as with the MCF-7 cells), I see a prominent band around 50kDa. What could be happening?
Reply With Quote
Reply

Tags
antibody , bands , cells , dr5 , mcf7 , multiple , trailr1


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
mouse hepatocytes isolation amicia Cell Biology and Cell Culture 39 05-11-2012 11:52 AM
Characteristics of cancerous cells aftabac Cell Biology and Cell Culture 2 08-26-2011 11:40 PM
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Production of Monoclonal Antibodies - Technique in Mouse Cellbiogal Antibody Forum 2 01-08-2009 10:52 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM


All times are GMT. The time now is 02:24 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14695 seconds with 16 queries