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| Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures. |
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| When performing a western blot on MCF-7 whole cell lysates I see a prominent band at around 37kDa and theree addl bands above it, the last one around 50kDa. However, when I run a 293 cell whole cell lysate (harvested the same way as with the MCF-7 cells), I see a prominent band around 50kDa. What could be happening? |
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| antibody , bands , cells , dr5 , mcf7 , multiple , trailr1 |
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