yes, it is possible I myself am doing a 270kDa protien.
Since this is the only protien that I have worked with in westerns so far... I will just give you the protocol that I use and you can make the comparison.
First, I use 7% Tris Acetate gels along with the respective running and transfer buffer(all come from Invitrogen
). These gels only require a 1hr run @150V. Next, the transfer is done for 2 HR @ 250mA with a PVDF membrane. I usually put an ice pack in box to keep it cool, but you can also do in cold room. Then, I do the regular washes, blocking, and antibody incubations specific to my protien.