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Which is better? Film, Storm, or Chromogenic

Which is better? Film, Storm, or Chromogenic - Western Blot Forum

Which is better? Film, Storm, or Chromogenic - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 01-14-2010, 07:47 PM
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Default Which is better? Film, Storm, or Chromogenic



What are the thoughts on which is the best method for viewing results and publication of the results?

Thanks
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Old 01-15-2010, 03:11 PM
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Default Re: Which is better? Film, Storm, or Chromogenic

Film is more sensitive than other options, but is probably the worst for publication in terms of quality. Chromagenic can be scanned/imaged nicely, but IMO is the least sensitive method available. Its also not super linear, so quantification is questionable. Storm is probably the easiest for publication purposes - you image directly into a computer, but not a lot of people have access to it, and it suffers from the down side that its not linear (not good for quantification) and is also less sensitive than film.

Bryan
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Old 01-18-2010, 05:32 PM
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Default Re: Which is better? Film, Storm, or Chromogenic

Thanks, I appreciate the response. I have access to Storm so I'm going to try to persuade my PI that we should use the Storm and not film.
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