Which is better? Film, Storm, or Chromogenic

[Serena Greene]

What are the thoughts on which is the best method for viewing results and publication of the results?

Thanks

[Warthaug]

Film is more sensitive than other options, but is probably the worst for publication in terms of quality. Chromagenic can be scanned/imaged nicely, but IMO is the least sensitive method available. Its also not super linear, so quantification is questionable. Storm is probably the easiest for publication purposes - you image directly into a computer, but not a lot of people have access to it, and it suffers from the down side that its not linear (not good for quantification) and is also less sensitive than film.

Bryan

[Serena Greene]

Thanks, I appreciate the response. I have access to Storm so I'm going to try to persuade my PI that we should use the Storm and not film.