| | Re: Centrifuge after sonication
As far as I know, Lysis buffer is going to break cell wall, so DNA will be free and then, it will be sonicated. The spin after sonication is usually a high-speed centrifugation during few minutes, which allows to precipitate all debris of your samples. It's like the last step to discard organelles, cell wall, etc.
If you want, you can add another step. After centrigufation, you can filter the sonicated sample through 45um syringe or miracloth membrane to get a clean lysate.
I hope it would be useful!