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Special blocking procedure/product for using mouse antibody on mouse tissue

Special blocking procedure/product for using mouse antibody on mouse tissue - Western Blot Forum

Special blocking procedure/product for using mouse antibody on mouse tissue - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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  #1  
Old 11-06-2009, 09:13 PM
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Default Special blocking procedure/product for using mouse antibody on mouse tissue



I am wondering if there is something to help use antibody raised in mouse on mouse tissues in Western Blot analysis?

I have to use a particular antibody and I am getting a lot of background and I think it's probably because of the same species.
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Old 12-05-2009, 12:58 AM
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Default Re: Special blocking procedure/product for using mouse antibody on mouse tissue

didn't know it was possible to raise mouse antibodies against mouse tissues...
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Old 12-20-2009, 05:33 PM
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Default Re: Special blocking procedure/product for using mouse antibody on mouse tissue

Hi there,

You have to use anti - mouse secondary antibody if there is mouse - primary antibody. Are you talking about your secondary antibody? I think you need an anti-mouse antibody, it's not possible to have mouse anti-mouse antibody so it would be an anti-mouse antibody raised in a different species, e.g. goat anti-mouse, rabbit anti-mouse, ect.
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Old 06-08-2012, 07:30 PM
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Default Re: Special blocking procedure/product for using mouse antibody on mouse tissue

I have a mouse anti-human antibody against a gene which is conserved between the two species. I am attempting to use the antibody against the mouse gene however I am also getting considerable background in my westerns. I've tried blocking using an unconjugated Fab fragment for mouse IgG however it doesn't seem to have eliminated all the background.
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Old 06-23-2012, 11:13 PM
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Default Re: Special blocking procedure/product for using mouse antibody on mouse tissue

I've had a similar issue when doing ICC. Lack of planning on my part and I had to use mouse ab on mouse tissue. Got terrible results with tons of bg. I would suggest finding another ab.
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