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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


protein stuck

protein stuck - Western Blot Forum

protein stuck - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 09-29-2009, 12:57 PM
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Question protein stuck



I have isolated total, nuclear and cytoplasmic proteins from mammalian cell culture stably expressing specific genes. qPCR analysis showed that there is an expression, but I have a western blot problem. I'm using polyclonal primary antibody against my 14 kDa protein. Although my marker run well, my protein samples got stuck at the interface between the stacking and the running gel. What is the problem? Any help would be appreciated.
Burcu
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