Hmmm we have this issue in the lab with sandwich assembly.
Most of the people assemble the sandwich in the transfer buffer - literally, they fill the microwave dish with 2-3 cm of buffer and assemble within.
I noticed, that when I do it this way I get with bunch of bubbles or just more blurry blots then I would assemble on the bench, even thought I would roll the sandwich very well with a roller. I guess I pick up the buffer when I lock the cassette.
How do you guys assemble sandwich? That is a bit of a silly question, but I am qurious what is the preferred way.