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Infected Cells Have Lighter Bands than Control

Infected Cells Have Lighter Bands than Control - Western Blot Forum

Infected Cells Have Lighter Bands than Control - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 09-09-2009, 04:10 PM
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Default Infected Cells Have Lighter Bands than Control



Hello everyone,

I have been having some problems with my western blots dealing with apoptosis. I'm focusing on isolating bands for caspase 9 in untreated cells and cells infected with Trichomonas vaginilis for 6 hours, however every time i run my western, the control bands are much darker than the infected. Before lysing, I run my infected cells through an apoptotic separator to isolate them. I have been running the gels with 20 micrograms of protein in each lane. Does anyone have any ideas why the infected cells have lighter bands than control? Thanks in advance.
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