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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Problem in Western blot

Problem in Western blot - Western Blot Forum

Problem in Western blot - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 08-21-2009, 03:33 AM
Pipette Filler
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Default Problem in Western blot



I loaded microsomes from LCC6MDR(which expresses G-GLycoprotein) to SDS-gel (Separating gel Acryamide%=7.5). I found that there were no bands near positions which represented its MW (170kDa). Most of the bands were in in the bottom of the gel.

I also did Western blot, and there were bands detected at the bottom. I wondered whether they were G-glycoprotein and I would like to know the reason why there were still signals detected in Western blot in this case.

Can anyone help me?

Thx!!!!
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Old 08-21-2009, 05:01 AM
Pipette Filler
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Default Re: Problem in Western blot

hi

you may try increasing the acrylamide concentration and reducing duration of time
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