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E. coli and S. aureus Western Blot
I'l be performing a western blot on bacterial samples after inducing a heat shock response. Any suggestions as to proteins I can target with antibodies for loading controls (internal controls)?
I've come across Sortaste transpeptidase for S. aureus.
Furthermore (I'm new to proteomics) is a bradford assay entirely necessary prior to western blot?
Re: E. coli and S. aureus Western Blot
Hi, I'm new in Western blot
I'm conducting western blot for heat shock too, and no ideas what I should use for the control.
What did you use for controls?
|aureus , bacteria , blot , bradford assay , coli , e. coli , s. aureus , western , western blot|
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|internal control of western blot for E. coli||lattee||Western Blot Forum||0||07-30-2009 03:33 AM|