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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


E. coli and S. aureus Western Blot

E. coli and S. aureus Western Blot - Western Blot Forum

E. coli and S. aureus Western Blot - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 08-05-2009, 07:32 PM
Pipette Filler
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Default E. coli and S. aureus Western Blot



I'l be performing a western blot on bacterial samples after inducing a heat shock response. Any suggestions as to proteins I can target with antibodies for loading controls (internal controls)?

I've come across Sortaste transpeptidase for S. aureus.

Furthermore (I'm new to proteomics) is a bradford assay entirely necessary prior to western blot?

Regards,
- tyler
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Old 01-08-2013, 10:03 PM
Pipette Filler
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Default Re: E. coli and S. aureus Western Blot

Hi, I'm new in Western blot

I'm conducting western blot for heat shock too, and no ideas what I should use for the control.
What did you use for controls?

Thank you
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aureus , bacteria , blot , bradford assay , coli , e. coli , s. aureus , western , western blot


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