Hello there, looking at your gel I think you have loading issues and possibly gel or even transfer issues.
Are you loosing any sample while loading (is it puffing out of the well?) - you could try increasing your sucrose.
How does your ladder look - are the bands quite sharp and well balanced in darkness? You may need to mix your SDS-PAGE gel a bit better and use a stacking gel as I see your bands are a bit unformed.
During transfer, you may have small bubbles i see missing spots in your bands. Try to get rid of bubbles with a roller or broken plastic pipette.
Let us know how your progress goes please post your next gel image / blot image.