All of a sudden my WB has stopped working only for anti-rabbit antibodies:
I checked the following:
1. THe transfer is fine
2. All mouse antibodies showing good signals
3. However rabbit antibodies do not show any signal even for abundant target proteins
4. Used tested primary and secondary rabbit ab, tried even brand new arrival of both primary and secondary rabbit antibodies.
5. The target protens are all expressed in the cell-lines I tested.
6. The ECL substrate is brand new and worked great for mouse
7. All conditions same but rabbit antibodies just WONT work.
Can anyone please explain this sad happening in my life?