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05-20-2009, 03:48 AM
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 stacking gel % Ya, so I was just going through the Bio-Rad Mini-Protean manual. They are giving recipes for PAGE according to acrylamide %, which are the same (same proportion for everything) for separating and stacking gels, you just substitute the running buffer for sthe stacking buffer. Why use different percentagesf or the stacking gel? Does one have to match % between stacking and separting gel?  |
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07-08-2009, 01:50 PM
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| Re: stacking gel % Thanks... but others in our lab and I used to do a fixed % (something like 10%) stacking gel which resulted in (some) successful blots. Anyway I try adjusting the stacking gel % accordingly the resolving gel now just in case... |
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