Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Western Blot Forum
Register Search Today's Posts Mark Forums Read

Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Tris in Laemmli buffer

Tris in Laemmli buffer - Western Blot Forum

Tris in Laemmli buffer - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 05-16-2009, 11:56 PM
Pipette Filler
Points: 657, Level: 13 Points: 657, Level: 13 Points: 657, Level: 13
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2009
Posts: 27
Thanks: 0
Thanked 7 Times in 6 Posts
Default Tris in Laemmli buffer



What are the advantages and disadvantages of adding Tris-Base (pH adjusted at 6.8 with HCl)?

And Some suggest to add B-mercaptoethanol fresh to Laemmli, i.e. you have have your Laemmli stock prepared to wich you add B-mercaethanol whenever you use some? Do you have to readjust pH then?

I was told that there was no difference in using a traditional Laemmli (SDS sample) buffer and LDS sample (NuPAGE) with SDS-PAGE (with my protein at least).What's the difference between a SDS Sample buffer and LDS Sample buffer? : Don't tell me SDS vs LDS, I mean what does it change?
Reply With Quote
  #2  
Old 06-26-2009, 08:23 PM
Pipette Filler
Points: 2, Level: 1 Points: 2, Level: 1 Points: 2, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2009
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Tris in Laemmli buffer

Hi-
I believe that LDS is better under cold conditions (i.e., won't precipitate as easily as SDS).
-joe
Reply With Quote
  #3  
Old 07-08-2009, 01:23 PM
Pipette Filler
Points: 657, Level: 13 Points: 657, Level: 13 Points: 657, Level: 13
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2009
Posts: 27
Thanks: 0
Thanked 7 Times in 6 Posts
Default Re: Tris in Laemmli buffer

Thanks
Reply With Quote
Reply

Tags
buffer , laemmli , tris


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
SDS-PAGE Protocol moleculardude Protein Science 14 02-12-2010 11:27 AM
Laemmli buffer admin Article Discussion 1 02-09-2010 01:48 AM
laemmli buffer dilution raya Western Blot Forum 3 09-23-2009 04:39 PM
Production of Monoclonal Antibodies - Technique in Mouse Cellbiogal Antibody Forum 2 01-08-2009 10:52 PM
Buffer Tris Question!!! please help! olenka Biochemistry Forum 2 09-18-2008 01:48 PM


All times are GMT. The time now is 07:32 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12558 seconds with 16 queries