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#1
05-15-2009, 04:52 AM
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 nice tight and dark non specific band I've been trying to blot AMPK a1 & a2 for a little while. my blots have not been a success: multi band mostly ... lately it looks better... but only until you look for molecular weight. Yes, I get a nice tight and dark band, but quite a bit too high (90-100), and identical between my WT and KO. Meanwhile, I get a pale band only with WT at 63kDa , this must be AMPKa1. What might be happening at 90-100 kDa? as my blots show les bands, that weird intruder band is getting darker?  |
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| band , dark , nice , specific , tight |
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